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Journal article
Selection and Implementation of Probiotics Saccharomyces Spp. Kb-05 and Saccharomyces Spp. Kb-08 Isolated from Buffalo Ruments to Increase the Nutritional Value of Rice Bran
I Gst. Nym. Gde Bidura Ni Wayan Siti
Volume : 34 Nomor : 2 Published : 2017, December
Journal of Biological and Chemical Research
Abstrak
The present study was conducted to find isolates Saccharomyces spp isolated from buffalo rumen which has cellulolytic activity (CMC-ase) and potential as a source of probiotics agents to increase the nutritional value of rice bran. The study included the isolation of yeast Saccharomyces spp from buffalo rumen, then both test enzyme activity (CMC-ase) and test as probiotic agent (passing test of several temperature levels, pH, acid and bile salt, and assimilate cholesterol). The superior isolate further tested its ability to increase the nutritional value of rice bran. Six isolates of Saccharomyces spp were isolated and selected from buffalo rumen as probiotics and had CMC-ase activity too. The six isolates, namely Saccharomyces spp.Kb-05 isolates; S.spp.Kb-08; S.spp.Kb-09; S.spp.Kb-10; S.spp.Kb-13; and S.spp.Kb-14, respectively. The use of both Saccharomyces spp.Kb-05 or Saccharomyces spp.Kb-08 isolate for rice bran fermentation were significantly (P<0.05) increased the crude protein and gross energy (GE) content of rice bran. Protein digestibility of rice bran that has been fermented by both isolates S.spp.Kb-05 and S. spp.Kb-08 were increased than control. Similarly, there was also an increase in the digestibility of crude fiber from fermented rice bran. It can be concluded that the fermentation of rice bran by both Saccharomyces spp.Kb-05 and S.spp.Kb-08 isolated from buffalo rumen can increase the energy, protein, and metabolizable energy of rice bran.